Abstract: Objective  To investigate the expression of miRNA141 in diffuse large B cell lymphoma (DLBCL) and its relationship with prognosis. Methods  Paraffin embedded tissue specimens of 40 patients with DLBCL admitted to the First Affiliated Hospital of Jinzhou Medical University from January 2017 to December 2019 before initial treatment were selected. The expression level of miRNA141 was detected by fluorescence realtime quantitative PCR. At the same time, the relationship between the relative expression level of miRNA141 and the clinical characteristics was investigated according to the groups of immune typing and international prognostic index (IPI score).In addition,  we selected paraffin tissue samples from 20 patients with reactive hyperplasia of lymph node(RHL) matched by gender and age as the control group. Results   The expression level of miRNA141 in DLBCL patients was lower than that in RHL group(P<0.05). The 2year overall survival rate (92.2%) of the group with high expression of miRNA141 in DLBCL was higher than 26.2% of the group with low expression(P=0.001). Multivariate COX model  analysis showed that  the low expression group of  miRNA141 (P=0.009) and the international prognostic index IPI≥3 (P=0.012) were the independent prognostic factors  among 40 cases of DLBCL. Conclusion  The expression of miRNA141 is decreased in patients with DLBCL,  and the overall survival rate of the group with high expression of miRNA141 was significantly higher than that of the group with low expression,  suggesting that miRNA141 may be another marker for assisting diagnosis and evaluating prognosis.

Key words: miRNAs, lymphoma, Bcell, miRNA141