Abstract: Objective  To explore the significance of both the IMN diagnosis and the evaluation of the disease by detecting the serum PLA2R and THSD7A antibody levels in IMN patients. Methods  A total of 137 patients who were diagnosed with renal biopsy in the third hospital of Hebei medical university from September  2016  to December 2017 were selected. Among them,  114 patients with IMN were treated as experimental group,  and the rest patients, including (7 cases of Vtype lupus nephritis and 16 cases of IgA nephropathy) as control group. The serum PLA2R and THSD7A antibody titer were determined by enzymelinked immuno sorbent assay (ELISA),  then the relationship between them and clinical indicators were analyzed. Results  ①The positive rate of serum PLA2R antibody in IMN patients is 57.0% (65/114). Vtype lupus nephritis and IgA nephropathy are negtive; the difference has been statistically significant (P＜0.05). The positive rate of serum THSD7A antibody in IMN patients is 9.6% (11/114),  Vtype with lupus nephritis and IgA nephropathy THSD7A antibody are negtive, the difference is statistically significant. The double positive rate in IMN group is 4.4% (5/114). ② In the IMN group,  24 h urine protein is quantitatively 9.4(4.9~14.0) g/d and blood albumin (21.2±3.7) g/L. PLA2R antibody positive 24 h urine protein quantification ［4.9(0.914.2)］  g/d,  blood albumin (24.4±5.6) g/L; The serum albumin (25.5±6.6) g/L is found in patients with positive THSD7A antibody 7.1(2.1~14.0) g/d. The results showed that there are statistically significant differences between the serum albumin and the serum albumin (P<0.05), so are the differences in blood albumin (P<0.05); while the quantitative difference of 24 h urine protein in patients with positive THSD7A antibody (P＞0.05) are of no statistical difference. ③There is a positive correlation between PLA2R antibody and 24 h urine protein (r=0.318,P=0.001),  which is negatively correlated with serum albumin level (r=-0.207, P=0.027).Conclusion  PLA2R and THSD7A antibody are specific indicators for the diagnosis of IMN. PLA2R antibody can assess the patient's condition; the urinary protein of the patients is high while their albumin is low, with double antibody being positive; However, whether THSD7A can assess the patient's condition and whether the combination can better predict the patient's condition remains to be further studied.

Key words: glomerulonephritis, , membranous; , Mtype phospholipase A2 receptor； Thrombospondin type1 domaincontaining 7A