Clinical Focus ›› 2015, Vol. 30 ›› Issue (6): 674-676677.

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Establishment and evaluation of real-time fluorescence PCR with DNA melting curve for detection of HLA-B27

Wen Haifeng;Feng Zhongjun;Han Wenlong;Qin Jin;Yu Wenjing   

  • Online:2015-06-05 Published:2015-06-05

Abstract: Objective To establish and evaluate the technique of the real-time fluorescence quantitative polymerase chain reaction with DNA melting curve analysis for detecting the HLA-B27.Methods Three kinds of methods,including melting curve analysis,micro-compleme

Key words: spondylitis;ankylosing, HLA-B27 antigen, polymerase chain reaction

CLC Number: