Relationship between RacGAP1 expression in gastric cancer and Helicobacter pylori L-type infection

doi:10.3969/j.issn.1004-583X.2016.01.012

Abstract

Abstract: Objective To explore the expression of RacGAP1 in gastric cancer tissue and its relationship with Helicobacter pylori L-type (Hp-L) infection.Methods A total of 125 cases of patients with gastric cancer were divided into Hp-L infection positive group (infection group) and Hp-L infection negative group (non-infection group), another 30 cases who had normal gastric mucosa and Hp-L non-infection were selected as control group. Immunohistochemical method was used to check the expression of Racgap1 protein in gastric cancer tissue. The Racgap1 mRNA expression was detected using RT-PCR method. All the patients were taken a period of five years of follow-up.Results RacGAP1 protein was expressed in nucleus mainly, and the positive rate of RacGAP1 expression in infection group and non-infection group were significantly higher than that of control group(P＜0.01), and the positive rate of RacGAP1 expression was significantly higher in infection group than in non-infection group(P＜0.05). RacGAP1 protein expression in gastric cancer tissues was associated with tumor size, invasion depth, lymph node metastasis, TNM staging and Hp infection. The survival rate of the non-infected group was significantly higher than that of the infected group (P<0.05).Conclusion RacGAP1 expression was significantly increased in gastric cancer tissues, Hp infection may be the reason leading to the increase of RacGAP1 expression, and the prognosis in patients with high expression of RacGAP1 was poor.

Key words: stomach neoplasms, helicobacter pylori, immunohistochemistry, reverse transcriptase polymerase chain reaction

CLC Number:

R735.2

Lu Ming,Liu Hengchang,Wang Quan. Relationship between RacGAP1 expression in gastric cancer and Helicobacter pylori L-type infection[J]. Clinical Focus, 2016, 31(1): 48-52.

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URL: https://huicui.hebmu.edu.cn/EN/10.3969/j.issn.1004-583X.2016.01.012

https://huicui.hebmu.edu.cn/EN/Y2016/V31/I1/48

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