Clinical Focus ›› 2021, Vol. 36 ›› Issue (8): 691-695.doi: 10.3969/j.issn.1004-583X.2021.08.003

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Analysis of norovirus genotypes of patients with acute gastroenteritis in Mentougou District of Beijing from 2018 to 2020

Liu Haitao(), Cao Dianqi, Zhang Bowen, Lyu Qiuyan, Su Jian   

  1. Mentougou District Centers for Disease Control and Prevention, Beijing 102300, China
  • Received:2021-07-21 Online:2021-08-20 Published:2021-08-30
  • Contact: Liu Haitao E-mail:283534279@qq.com

Abstract:

Objective To investigate the norovirus genotypes of patients with acute gastroenteritis in Mentougou District of Beijing. Methods Ribonucleic Acid(RNA) of norovirus was detected in fecal samples of acute gastroenteritis patients by real time fluorescent quantitative PCR, and region of polyzyme and capsid protein of type samples of norovirus GⅡ was amplified by general RT-PCR, in order to determine sequence of amplified. Results Positive rate of norovirus was 10.75%, 95.89% for GⅡ gene group and 4.11% for G Igene group were detected. GⅡ gene of norovirus include GⅡ.6 [P7], GⅡ.4 [P16], GⅡ.3 [P12], GⅡ.2 [P16] and GⅡ.17 [P17], variation of each genotype virus was not obvious, and nucleotide homology was 86%-100%. Conclusion There are many genotypes and different recombinant subtypes of norovirus in Mentougou District of Beijing, continuous monitoring of dynamic epidemic characteristics of norovirus recombinant genotypes is helpful to improve ability of early warning for disease prevention and control.

Key words: norovirus, gastroenteritis, genotype

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