Abstract: ObjectiveTo analyze the epidemiology， antibiotic resistance， and antiinfection treatment of hospital infection with gram positive bacteria from patients who had hematological diseases during 20052014. MethodsA total of 827 gram positive bacterial strains were isolated from patients with hematological diseases from January 2005 to December 2014. Antimicrobial susceptibility testing was done by microdilution method. Results①The percentage of isolated gram positive bacterial strains from secretion and urine was higher than that of gram negative bacillus， while the percentage of isolated gram positive bacterial strains from sputum was lower than that of gram negative bacillus; The percentage of isolated gram positive bacterial strains showed upward during  20052007， and then downward， but upward again after 2012; The staphylococcus presented an upward trend， while the enterococcus presented an upward trend; In Staphylococcus aureus， the positive rate of MASA was lower than that of our hospital. ②All the isolated gram positive bacterial strains were sensitive to vancomycin， teicoplanin and linezolid， while the resistance rate to moxifloxacin with an upward trend in staphylococcus aureus and staphylococcus epidermidis， and there was no obvious change in Enterococcus faecalis and Enterococcus faecium. ③The rate of catheterrelated gram positive strains in all gram positive bacterial strains was significantly higher than the rate of catheterrelated gram negative bacillus in all gram negative bacillus (P<0.05). ④No significant difference in efficacy occurred between antibiotics to gram negative bacillus combined with or without antibiotics to gram positive bacteria when used in patients with fever and lack of granulocyte (P＞0.05). ConclusionOur research summarized the epidemiology， antibiotic resistance of hospital infection with gram positive bacteria， and it is valuable for the choice of rational antimicrobial therapy.

Key words: hematological disease, infection, grampositive bacteria