Abstract: ObjectiveTo analyze the diagnostic value of highfrequency color Doppler ultrasonography and hepatobiliary seintigraphy in early diagnosis of biliary atresia. MethodsThe study involved 56 neonates and infants with cholestatic jaundice who were confirmed with cholangiography by operation or abdominoscopy. Preoperative highfrequency color Doppler ultrasonography and hepatobiliary seintigraphy were performed. Two diagnostic methods were compared with the gold standards.The sensitivity， specificity， accuracy， positive predictive value and negative predictive value were calculated. ResultsIn  56 consecutive cholestatic infants(32 boys，24 girs)， there were 34 infants with biliary atresia (60.7%) and 22 infants with nonbiliary atresia (39.3%). The sensitivity， specificity， accuracy， positive predictive value and negative predictive value of biliary atresia diagnosis by highfrequency color Doppler ultrasonography were 88.2%，77.3%，83.93%，85.71%，80.95%， and those of biliary atresia diagnosis by hepatobiliary seintigraphy were 100.0%，63.6%，85.7%，81.0% and 100.0%，respectively. ConclusionHighfrequency color Doppler ultrasonography diagnosis of biliary atresia with high specificity，superior to that of hepatobiliary seintigraphy; Hepatobiliary seintigraphy diagnosis of biliary atresia with high sensitivity， superior to that of highfrequency color Doppler ultrasonography. The combination of two methos can distinguish between biliary atresia and other causes of neonates and infants cholestasis with high accuracy，and unnecessary surgery may be avoided， improved clinic prognostic.

Key words: jaundice；biliary atresia；diagnostic method;ultrasonography, Doppler, color；radionuclide imaging