关键词: 肺炎, 病毒性；巨细胞病毒；细胞活素类；流式细胞术

Abstract: Objective  To study diagnosis and clinical significance of neutrophil surface adhesion molecules including CD11b from cytomegalovirus (CMV) infection pneumonia in children. Methods  Blood samples were collected from 113 children of CMV infection pneumonia including male 80 and female 40 cases between November 2014 and October 2016. Healthy male 21 cases and female 12 cases served as healthy control group from outpatients in the same period. Children of CMV infection pneumonia were divided into high active CMV infection group， CMV infection group and the negative control group， and healthy group as normal control group according to CMVpp65 antigen detection. Neutrophil surface adhesion molecule CD11b was analyzed by flow cytometry and compared respectively. ROC curve of CD11b in children of CMV infection pneumonia was made. Results  The expression ratio of CD11b in high active CMV infection group was significantly different as compared with negative control group and control group (P<0.05); optimal cutoff value of CD11b in high active CMV infection group was 40.11%， the sensitivity was 100%， the specificity in high active CMV infection group was 63.19%， and the area under the ROC was 0.612. The optimal cutoff value of CD11b in CMV infection group was 63.87%， the sensitivity of CD11b was 92.87%， the specificity of CD11b was 98.34%， and the area under of ROC of CD11b was 0.991. Conclusion  The neutrophils CD11b expression in children of CMV infection pneumonia is down regulation. CD11b expression can be used as a laboratory diagnosis basis of children’s CMV infection.

Key words: pneumonia, viral;cytomegalovirus, cytokines, flow cytometry