Six1在结肠癌组织高表达并增强结肠癌细胞侵袭能力

doi:10.3969/j.issn.1004-583X.2019.05.010

临床荟萃

Six1在结肠癌组织高表达并增强结肠癌细胞侵袭能力

1.国营长风机器厂职工医院消化内科，甘肃兰州 730070；2.甘肃省人民医院消化科，甘肃兰州 730000

出版日期:2019-05-20 发布日期:2019-06-28
通讯作者: 通信作者：魏小果，Email: 1322273042@qq.com

Six1 was highly expressed in colon carcinoma patients and enhanced the invasive ability of colon cancer cells

1.Department of Digestive Medicine, Staff and Workers Hospital, Changfeng Machinery Factory,
Lanzhou 730070， China；2.Department of Digestive, Gansu Provincial Hospital, Lanzhou 730000， China

Online:2019-05-20 Published:2019-06-28
Contact: Corresponding author: Wei Xiaoguo, Email: 1322273042@qq.com

摘要/Abstract

摘要： 目的探讨同源异形框基因Six1（sine oculis homeobox homolog1）在结肠癌组织中的表达及其对结肠癌细胞侵袭能力的影响。方法免疫组织化学法检测90例结肠癌及癌旁正常组织中 Six1的蛋白阳性表达率。以Six1小分子干扰RNA（siRNA）沉默Six1，应用Transwell小室实验检测细胞侵袭力。结果 Six1蛋白在结肠癌组织中的阳性表达率高于其在癌旁正常组织中的阳性表达率。沉默Six1后，Six1 mRNA和Six1蛋白表达水平显著降低，HT29细胞穿膜细胞数也明显降低。结论 Six1表达与结肠癌发展密切相关，抑制Six1的表达可抑制结肠癌细胞的侵袭能力。

关键词: Six1, 结肠肿瘤, 迁移

Abstract: Objective To investigate the expression of sine oculis homeobox homolog1 (Six1) in colon cancer tissues and its effect on cell invasion of colon cancer cells. Methods The positive expression rate of Six1 in 90 cases of colon cancer and adjacent normal tissues were detected by immunohistochemistry method. Six 1 small interfering RNA (SiRNA) was applied to suppress Six1. The invasion ability of colon cancer cells was detected by Transwell chamber test. Results The positive rate of Six1 protein in colon cancer tissue was higher than that of normal tissues adjacent to cancer. After suppressing Six1, Six1 mRNA and protein expression levels of Six1 were significantly decreased, and the invasion cell number of HT29 cells was significantly decreased. Conclusion The expression of Six1 is closely related to the development of colon cancer, and inhibition of Six1 expression can prevent the invasion of colon cancer cells.

Key words: six1, colonic neoplasms, invasion

马晓峰1，马莉1，魏小果2. Six1在结肠癌组织高表达并增强结肠癌细胞侵袭能力[J]. 临床荟萃, doi: 10.3969/j.issn.1004-583X.2019.05.010.

Ma Xiaofeng1, Ma Li1, Wei Xiaoguo2. Six1 was highly expressed in colon carcinoma patients and enhanced the invasive ability of colon cancer cells[J]. Clinical Focus, doi: 10.3969/j.issn.1004-583X.2019.05.010.

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链接本文: https://huicui.hebmu.edu.cn/CN/10.3969/j.issn.1004-583X.2019.05.010

https://huicui.hebmu.edu.cn/CN/Y2019/V34/I5/426

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Six1在结肠癌组织高表达并增强结肠癌细胞侵袭能力

Six1 was highly expressed in colon carcinoma patients and enhanced the invasive ability of colon cancer cells

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