Expression of PD-1 on T-lymphocytes in acute leukemia after allogeneic hematopoietic stem cell transplantation

doi:10.3969/j.issn.1004-583X.2021.03.013

Abstract

Abstract:

Objective To explore the dynamic change and clinical significance of PD-1 (programmed cell death protein 1) expression on T-lymphocytes in acute leukemia after allogeneic hematopoietic stem cell transplantation (allo-HSCT). Methods Twenty-three healthy volunteers (the control group) and 40 patients with acute leukemia (the transplantation group) were selected, peripheral blood samples were collected 1, 2, 3, 4, 5, 6 months after allo-HSCT, and PD-1 expression levels on CD3⁺CD4⁺ and CD3⁺CD8⁺ T-lymphocytes were tested by the flow cytometry. The differences of PD-1 expression levels between the allo-HSCT group and the control group were analyzed. Results The expression levels of PD-1 on CD3⁺CD4⁺ and CD3⁺CD8⁺ T-lymphocytes in acute leukemia patients in the transplantation group at 1 and 2 months after allo-HSCT were higher than those of the control group. The PD-1 expression levels on CD3⁺CD4⁺ T-lymphocyte in the transplantation group were higher than those of the control group at 3, 4, 5 and 6 month after allo-HSCT, but they showed a downtrend with the transplantation time, while the PD-1 expression levels in CD3⁺CD8⁺ T-lymphocyte had no obvious statistical significance (P>0.05 for all). Conclusion The PD-1expression levels on CD3⁺CD4⁺ T-lymphocyte in patients with acute leukemia after allo-HSCT were higher than those normal levels at the early stage and showed a downtrend with the transplantation time; while the PD-1 expression levels on CD3⁺CD8⁺ T-lymphocyte were increased transiently at the early stage after allo-HSCT.

Key words: leukemia, programmed cell death protein 1, hematopoietic stem cell transplantation, lymphocyte

CLC Number:

R733.7

Liu Jianning, Sun Li, Niu Zhiyun, Wen Shupeng, Wang Ying, Zhang Xuejun, Wang Fuxu. Expression of PD-1 on T-lymphocytes in acute leukemia after allogeneic hematopoietic stem cell transplantation[J]. Clinical Focus, 2021, 36(3): 251-255.

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URL: https://huicui.hebmu.edu.cn/EN/10.3969/j.issn.1004-583X.2021.03.013

https://huicui.hebmu.edu.cn/EN/Y2021/V36/I3/251

Figures/Tables 3

References 22

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预处理方案	例数	药物	剂量	时间(d)
mBu/Cy	5	Ara-c	2 g/m²	-9
		Bu	3.2 mg/(kg·d)	-8~-6
		Cy	1.8 g/(m²·d)	-5~-4
		MeCCNU	250 mg/m²	-3
mBu/Cy+ATG	17	Ara-c	4 g/(m²·d)	-10~-9
		Bu	3.2 mg/(kg·d)	-8~-6
		Cy	1.8 g/(m²·d)	-5~-4
		ATG	2.5 mg/(kg·d)	-5~-2
Bu/Cy+FLAG	1	Ara-c	4 g/(m²·d)	-10~-9
		Bu	3.2 mg/(kg·d)	-8~-6
		Cy	40 mg/(kg·d)	-5~-4
		Flu	30 mg/(m²·d)	-6~-2
		G-CSF	5 μg/kg
BU/Cy+FLAG+ATG	3	Ara-c	4 g/(m²·d)	-10~-9
		Bu	3.2 mg/(kg·d)	-8~-6
		Cy	40 mg/(kg·d)	-5~-4
		Flu	30 mg/(m²·d)	-6~-2
		G- CSF	5 μg/kg
		ATG	2.5 mg/(kg·d)	-5~-2
TBI/Cy	4	TBI	4 Gy/d	-5~-4
		Cy	60 mg/(kg·d)	-3~-2
TBI/Cy+ATG	7(其中3例加依托泊苷处理)	Cy	1.8 g/(m²·d)	-5~-4
		ATG	2.5 mg/(kg·d)	-5~-2
		TBI	4 Gy/d	-2~-1
TBI/Cy+FLAG+ATG	3	Ara-c	2 g/(m²·d)	-7~-4
		TBI	4 Gy/d	-9~-8
		Cy	40 mg/(kg·d)	-3~-2
		Flu	30 mg/(m²·d)	-7~-4
		ATG	2.5 mg/(kg·d)	-5~-2
		G-CSF	5 μg/kg	-7~-4

预处理方案	例数	药物	剂量	时间(d)
mBu/Cy	5	Ara-c	2 g/m²	-9
		Bu	3.2 mg/(kg·d)	-8~-6
		Cy	1.8 g/(m²·d)	-5~-4
		MeCCNU	250 mg/m²	-3
mBu/Cy+ATG	17	Ara-c	4 g/(m²·d)	-10~-9
		Bu	3.2 mg/(kg·d)	-8~-6
		Cy	1.8 g/(m²·d)	-5~-4
		ATG	2.5 mg/(kg·d)	-5~-2
Bu/Cy+FLAG	1	Ara-c	4 g/(m²·d)	-10~-9
		Bu	3.2 mg/(kg·d)	-8~-6
		Cy	40 mg/(kg·d)	-5~-4
		Flu	30 mg/(m²·d)	-6~-2
		G-CSF	5 μg/kg
BU/Cy+FLAG+ATG	3	Ara-c	4 g/(m²·d)	-10~-9
		Bu	3.2 mg/(kg·d)	-8~-6
		Cy	40 mg/(kg·d)	-5~-4
		Flu	30 mg/(m²·d)	-6~-2
		G- CSF	5 μg/kg
		ATG	2.5 mg/(kg·d)	-5~-2
TBI/Cy	4	TBI	4 Gy/d	-5~-4
		Cy	60 mg/(kg·d)	-3~-2
TBI/Cy+ATG	7(其中3例加依托泊苷处理)	Cy	1.8 g/(m²·d)	-5~-4
		ATG	2.5 mg/(kg·d)	-5~-2
		TBI	4 Gy/d	-2~-1
TBI/Cy+FLAG+ATG	3	Ara-c	2 g/(m²·d)	-7~-4
		TBI	4 Gy/d	-9~-8
		Cy	40 mg/(kg·d)	-3~-2
		Flu	30 mg/(m²·d)	-7~-4
		ATG	2.5 mg/(kg·d)	-5~-2
		G-CSF	5 μg/kg	-7~-4

时间	PD-1/CD3⁺CD4⁺ T	PD-1/CD3⁺CD8⁺ T
移植后1个月	49.39±22.97	38.27±21.12
移植后2个月	43.75±26.75	28.51±20.69
移植后3个月	26.92±22.25	18.79±19.70
移植后4.5个月	28.59±19.83	17.12±18.83
移植后6个月	25.26±16.12	11.64±16.67
F值	5.082	8.492
P值	0.002	0.002

时间	PD-1/CD3⁺CD4⁺ T	PD-1/CD3⁺CD8⁺ T
移植后1个月	49.39±22.97	38.27±21.12
移植后2个月	43.75±26.75	28.51±20.69
移植后3个月	26.92±22.25	18.79±19.70
移植后4.5个月	28.59±19.83	17.12±18.83
移植后6个月	25.26±16.12	11.64±16.67
F值	5.082	8.492
P值	0.002	0.002