Abstract: Objective  To investigate the effects of female healthy donor characteristics on the components of hematopoietic cells and immune cells from a mixture of bone marrow and peripheral blood collection preactivated by granulocyte colonystimulating factor (GCSF). Methods  A total of 111 healthy female donors were mobilized with GCSF to determine the number of CD34+ cells and Tcell subsets in a mixture of bone marrow and peripheral blood by flow cytometry. The effects of pregnancy,  age,  height and other donor characteristics on the cell components in bone marrow and peripheral blood collection were analyzed.Results  The median values of CD34+cells,  CD3+T cells,  CD3+CD4+T cells,  CD3+CD8+T cells and CD3+CD4-CD8- regulatory T cells in the mixture of bone marrow and peripheral blood samples from 111 healthy female donors were 2.39×106,  226.57×106,  120.80×106,  89.99×106 and 15.05×106 per kg of donor body weight,  respectively.① Age had an effect on the number of CD3+CD4-CD8-T cells in the mixed allografts.② Body mass index (BMI) had an effect on the number of CD3+T cells and CD3+CD4-CD8-T cells in the mixed collection.③The number of CD3+T cells,  CD3+CD4+T cells and CD3+CD8+T cells in the mixture was affected by lymphocyte (LYM) counts on the day of peripheral blood stem cell collection.④The number of CD3+T cells and CD3+CD4-CD8-T cells in the mixture was affected by lymphocyte counts on the day of bone marrow stem cell collection.⑤Compared with pregnant donors, the mixed collection of non pregnant donors contained a higher number of CD3+CD8+T cells. Conclusion  Donor age,  BMI, pregnancy status and LYM on the day of bone marrow stem cells and peripheral blood stem cells were the main influencing factors of bone marrow immunohistochemical components.

Key words: hematopoietic stem cell transplantation, granulocyte colonystimulating factor, living donor, body mass index, transplantation immunology