Effects of cerebroprotein hydrolysate on Nrf2 oxidative stress signaling pathway in acute stroke patients

doi:10.3969/j.issn.1004-583X.2021.11.007

Abstract

Abstract:

Objective To explore the effect of cerebroprotein hydrolysate on nuclear factor-E2 correlation factor 2-cored Keap1-Nrf2/ARE (Nrf2) oxidative stress signaling pathway in patients with acute stroke (AS). Methods Totally 195 AS patients treated in the hospital from October 2017 to October 2020 were divided into study group (n=98) and control group (n=97) according to therapeutic methods. The patients in control group were administered with conventional therapies involving the enhancement of cerebral circulation, improvement of brain function and antiplatelet aggregation, and patients in study group additionally with cerebroprotein hydrolysate on the basis of control group. All patients were treated for 14 days, the efficacy was observed, The key observation was National Institutes of Health Stroke Scale (NIHSS) score, Activity Ability-daily Life (Barthel) Index, inflammatory factors including tumor necrosis factor-α (TNF-α), interleukin-8(IL-8) and interleukin-19(IL-19) oxidative stress indexes including serum superoxide dismutase (SOD), malondialdehyde (MDA), vascular endothelial function such as serum adiponectin (APN), and plasma von Willebrand factor (vWF), protein expressions of Nrf2 oxidative stress signaling pathways Keap1, NQO1, ARE and Nrf2.Results Total effective rate in study group (91.84%) and control group (60.82%) was statistically significant difference (P<0.01). After treatment, NIHSS score were significantly lower in study group than in control group. Barthel index was significantly higher in study group than in control group (P<0.01). TNF-α, IL-8, IL-19, MDA, vWF and Keap1 were significantly lower in study group than in control group. Serum SOD, APN, NQO1, ARE and Nrf2 were significantly higher in study group than in control group(P<0.01). Conclusion For AS patients, cerebroprotein hydrolysate can effectively regulate Nrf2 oxidative stress signaling pathway related proteins, and deliver significant effects in improving the vascular endothelial function, controlling inflammation and oxidative stress, and enhancing the neurological function and quality of life.

Key words: stroke, brain protein hydrolysate, oxidative stress, signal pathway

CLC Number:

R743

Liu Ting, Ji Weidong, Yang Qingsong. Effects of cerebroprotein hydrolysate on Nrf2 oxidative stress signaling pathway in acute stroke patients[J]. Clinical Focus, 2021, 36(11): 996-1000.

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URL: https://huicui.hebmu.edu.cn/EN/10.3969/j.issn.1004-583X.2021.11.007

https://huicui.hebmu.edu.cn/EN/Y2021/V36/I11/996

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References 20

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组别	例数	基本控制	显效	有效	总有效
研究组	98	16(16.32)	51(52.04)	23(23.46)	90(91.84)
对照组	97	6(6.18)	25(92.59)	28(28.86)	59(60.82)
χ²值			7.819		26.010
P值			0.020		<0.01

组别	例数	基本控制	显效	有效	总有效
研究组	98	16(16.32)	51(52.04)	23(23.46)	90(91.84)
对照组	97	6(6.18)	25(92.59)	28(28.86)	59(60.82)
χ²值			7.819		26.010
P值			0.020		<0.01

组别	例数	NIHSS评分		Barthel指数
组别	例数	治疗前	治疗后	治疗前	治疗后
研究组	98	24.16±4.23	7.39±2.41^*	53.26±5.77	86.71±3.68^*
对照组	97	24.74±3.85	12.65±1.51^*	54.23±4.85	74.72±3.87^*
t值		1.001	18.242	1.270	22.172
P值		0.318	0.000	0.206	0.000

组别	例数	NIHSS评分		Barthel指数
组别	例数	治疗前	治疗后	治疗前	治疗后
研究组	98	24.16±4.23	7.39±2.41^*	53.26±5.77	86.71±3.68^*
对照组	97	24.74±3.85	12.65±1.51^*	54.23±4.85	74.72±3.87^*
t值		1.001	18.242	1.270	22.172
P值		0.318	0.000	0.206	0.000

组别	例数	TNF-α(μg/L)		IL-8(μmoL/L)		IL-19(ng/L)
组别	例数	治疗前	治疗后	治疗前	治疗后	治疗前	治疗后
研究组	98	16.15±5.29	7.39±2.13^*	29.87±6.27	15.88±3.71^*	81.13±28.77	22.87±4.19^*
对照组	97	16.38±4.85	12.46±3.25^*	29.55±5.39	21.77±4.28^*	80.51±28.15	37.65±8.33^*
t值		0.316	12.896	0.382	10.272	0.152	15.675
P值		0.752	0.000	0.703	0.000	0.879	0.000