RacGAP1在胃癌中的表达与幽门螺杆菌L型感染的关系

doi:10.3969/j.issn.1004-583X.2016.01.012

临床荟萃 ›› 2016, Vol. 31 ›› Issue (1): 48-52.doi: 10.3969/j.issn.1004-583X.2016.01.012

RacGAP1在胃癌中的表达与幽门螺杆菌L型感染的关系

路明¹, 刘恒昌², 王权²

1.吉林市中心医院基本外科,吉林吉林 132000;
2.吉林大学白求恩第一医院胃结直肠肛门外科,吉林吉林 130021

收稿日期:2015-08-24 出版日期:2016-01-05 发布日期:2016-04-19
通讯作者: 王权,Email:wangquan-jlcc@hotmail.com
基金资助:
卫生部医药卫生科技发展研究中心课题(W2013R63)

Relationship between RacGAP1 expression in gastric cancer and Helicobacter pylori L-type infection

Lu Ming¹,Liu Hengchang²,Wang Quan²

1.Department of Genenral Surgery, Central Hospital of Jilin, Jilin 132000, China;
2.Department of Gastrointestinal Surgery, the First Affiliated Hospital of Jilin University, Changchun 130021, China

Received:2015-08-24 Online:2016-01-05 Published:2016-04-19
Contact: Wang Quan,Email: wangquan-jlcc@hotmail.com

摘要/Abstract

摘要： 目的探讨胃癌组织中RacGAP1的表达情况,及其与幽门螺杆菌L型(Hp-L)感染的关系。方法选取125例胃癌患者,根据有无Hp-L感染分为Hp-L感染阳性组(感染组)和Hp-L感染阴性组(未感染组),另选取胃镜检查为正常胃黏膜,且Hp-L感染阴性的体检者30例作为对照组,应用免疫组织化学法检测标本中RacGAP1蛋白的表达情况,应用逆转录-聚合酶链反应(RT-PCR)法检测各组中RacGAP1 mRNA的表达情况,并对胃癌患者进行为期5年的随访。结果 RacGAP1主要表达在胃癌组织细胞核中,感染组和未感染组RacGAP1阳性率显著高于对照组(P＜0.01),感染组RacGAP 1阳性率显著高于未感染组(P＜0.05)。RacGAP1 mRNA的表达组间存在显著差异:感染组＞未感染组＞对照组;RacGAP1蛋白在胃癌组织中的表达与瘤体大小、浸润深度、淋巴转移、TNM分期和Hp感染有关;感染组生存率63.6%(49/77),未感染组生存率83.3%(30/36)。未感染组的生存率显著高于感染组(P＜0.05)。结论胃癌组织中RacGAP1表达显著升高,Hp感染可能导致RacGAP1表达的提高,且RacGAP1高表达的患者预后较差。

关键词: 胃肿瘤, 幽门螺杆菌, 免疫组织化学法, 逆转录聚合酶链反应

Abstract: Objective To explore the expression of RacGAP1 in gastric cancer tissue and its relationship with Helicobacter pylori L-type (Hp-L) infection.Methods A total of 125 cases of patients with gastric cancer were divided into Hp-L infection positive group (infection group) and Hp-L infection negative group (non-infection group), another 30 cases who had normal gastric mucosa and Hp-L non-infection were selected as control group. Immunohistochemical method was used to check the expression of Racgap1 protein in gastric cancer tissue. The Racgap1 mRNA expression was detected using RT-PCR method. All the patients were taken a period of five years of follow-up.Results RacGAP1 protein was expressed in nucleus mainly, and the positive rate of RacGAP1 expression in infection group and non-infection group were significantly higher than that of control group(P＜0.01), and the positive rate of RacGAP1 expression was significantly higher in infection group than in non-infection group(P＜0.05). RacGAP1 protein expression in gastric cancer tissues was associated with tumor size, invasion depth, lymph node metastasis, TNM staging and Hp infection. The survival rate of the non-infected group was significantly higher than that of the infected group (P<0.05).Conclusion RacGAP1 expression was significantly increased in gastric cancer tissues, Hp infection may be the reason leading to the increase of RacGAP1 expression, and the prognosis in patients with high expression of RacGAP1 was poor.

Key words: stomach neoplasms, helicobacter pylori, immunohistochemistry, reverse transcriptase polymerase chain reaction

中图分类号:

R735.2

路明, 刘恒昌, 王权. RacGAP1在胃癌中的表达与幽门螺杆菌L型感染的关系[J]. 临床荟萃, 2016, 31(1): 48-52.

Lu Ming,Liu Hengchang,Wang Quan. Relationship between RacGAP1 expression in gastric cancer and Helicobacter pylori L-type infection[J]. Clinical Focus, 2016, 31(1): 48-52.

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链接本文: https://huicui.hebmu.edu.cn/CN/10.3969/j.issn.1004-583X.2016.01.012

https://huicui.hebmu.edu.cn/CN/Y2016/V31/I1/48

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RacGAP1在胃癌中的表达与幽门螺杆菌L型感染的关系

Relationship between RacGAP1 expression in gastric cancer and Helicobacter pylori L-type infection

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