关键词: 干燥综合征, 水通道蛋白, 自身免疫疾病, 酶联免疫吸附法

Abstract: Objective  To analyze the expression level and clinical value of aquaporins (AQPs) in peripheral blood of patients with Sjogren's syndrome(SS).Methods  Twentythree SS patients of clinically confirmed were selected as experimental group. While 14 patients with autoimmune diseases (AIDs) were selected as experimental control group and 20 healthy subjects  were selected as healthy control group.The  expressions of AQP5 and AQP8 in peripheral blood were detected by enzymelinked immunosorbent assay (ELISA).Results  AQP5 in SS group was higher than that in healthy control group（Z=1.826, P=0.041）,  and there was no significant difference  in the level of AQP8（P＞0.05）. There was no significant difference in the expression levels of AQP5 and AQP8 between SS group and  experimental control group（P＞0.05）.The  level of AQP5  was correlated  with that of AQP8 in peripheral blood of SS patients（r=0.991, P＜0.01）. The levels of AQP5 （r=0.4805,  P=0.032） and AQP8（r=0.5357,  P=0.0149）  in peripheral blood of patients with SS correlated with peripheral blood IgM. And neither had relationship with  IgG,  IgA,  ESR,  CRP,  RF and leukocytes,  hemoglobin and platelets（P＞0.05）.The SS patients were separated  into  different subgroups by with or without interstitial lung disease,  with or without hematological involvement,  antiSSB antibody negative and positive,  and salivary gland pathological grade,  there were no significant differences in subgroup analysis of patients（P＞0.05）.Conclusion  The expression of AQP5 was higher in  SS patients than in healthy  people,  which may have  certain value for the diagnosis and  the studies for  pathogenesis of SS.The expression  level of AQP8  related to the level of AQP5 in SS patients.And their significance for SS need  further study.

Key words: Sjogren’s syndrome, aquaporins;autoimmune diseases, enzymelinked immunosorbent assay