基于重度抑郁症患者血清中miR-221-3p表达的相关研究

doi:10.3969/j.issn.1004-583X.2019.11.008

摘要/Abstract

摘要： 目的检测重度抑郁症患者血清中微小RNA2213p（miR2213p）的表达，分析其临床意义，探讨miR2213p与脑源性神经营养因子（BDNF）、人神经营养因子4（NT4）、S100B蛋白（S100B）和超氧化物歧化酶（SOD）的关系，以及血清与脑脊液中miR2213p表达的一致性。方法选择重度抑郁症患者59例作为观察组，留取正常血清标本，选择正常成人血清标本59例作为对照组。应用实时荧光定量PCR法（qPCR）检测两组中miR2213p的表达，应用酶联免疫吸附实验法（ELISA）检测观察组中BDNF、S100B的表达，应用黄嘌呤氧化酶法检测血清中SOD的表达，应用Western Blot检测血清中NT4的表达。对重度抑郁症中39例患者抽取脑脊液，检测脑脊液中miR2213p的表达。结果两组中miR2213p的表达差别有统计学意义（P<0.05），miR2213p在有无精神障碍家族史亚组中的差别有统计学意义（P<0.05）。miR2213p与BDNF（r=-0.51，P=0.036）、miR2213p与SOD（r=-0.57, P=0.013）、miR2213p与NT4（r=-0.62, P=0.009）呈负相关，miR2213p与S100B（r=0.59, P=0.014）呈正相关。miR2213p在血清和脑脊液中的表达呈正相关（r=0.72, P=0.001）。结论重度抑郁症患者血清miR2213p的异常表达参与病变的形成和进展，miR2213p可能通过对神经营养因子、损伤因子和应激指标的调控发挥作用。脑脊液中miR2213p的表达与血清中具有一致性。

关键词: 抑郁症, miR2213p, 脑源性神经营养因子, S100蛋白质类, 超氧化物歧化酶, 外周血, 脑脊液

Abstract: Objective To detect the expression of RNA2213p (miR2213p) in patients with severe depression, analyze its clinical significance, and explore the relationship between miR2213p and brainderived neurotrophic factor(BDNF), human neurotrophic factor4(NT4), S100B protein(S100B) and superoxide dismutase(SOD), respectively. And to explore the expression consistency of miR2213p in cerebrospinal fluid and serum. Methods A total of 59 patients with severe depression were selected as observation group and normal serum samples were taken. Totally 59 serum samples of normal adults were taken as control group. MiR2213p was detected by realtime fluorescence quantitative PCR(qPCR) in two groups. The expressions of BDNF and S100B were detected by ELISA method in observation group, SOD was detected by xanthine oxidase method in serum, and NT4 was detected by Western Blot method in the serum. MiR2213p was detected in cerebrospinal fluid which was extracted from 39 patients with severe depression. Results There was statistically significance in the difference of MiR2213p of two groups (P<0.05). miR2213p in the groups divided by family history of mental disorders also showed significant difference(P<0.05). Negative correlation was found between miR2213p and BDNF(r=-0.51, P=0.036), miR2213p and SOD (r=-0.57, P=0.013), miR2213p and NT4(r=-0.62, P=0.009). miR2213p and S100B had positive correlation(r=0.59, P=0.014). Positive correlation of miR2213p existed between serum and cerebrospinal fluid(r=0.72, P=0.001). Conclusion Abnormal expression of serum miR2213p affects the formation and progression of severe depression. MiR2213p may play a role in regulating neurotrophic factors, injury factors and stress indicators. There is expression consistency of miR2213p in the serum and cerebrospinal fluid.

Key words: depressive disorder, mir2213p, brainderived neurotrophic factor, s100 proteins, superoxide dismutase, peripheral blood, cerebrospinal fluid

吕睿a，曹业才b，王晓琪c. 基于重度抑郁症患者血清中miR-221-3p表达的相关研究[J]. 临床荟萃, doi: 10.3969/j.issn.1004-583X.2019.11.008.

Lu Ruia, Cao Yecaib, Wang Xiaoqic. Expression analysis of serum miR2213p in patients of severe depression[J]. Clinical Focus, doi: 10.3969/j.issn.1004-583X.2019.11.008.

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https://huicui.hebmu.edu.cn/CN/Y2019/V34/I11/999

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基于重度抑郁症患者血清中miR-221-3p表达的相关研究

Expression analysis of serum miR2213p in patients of severe depression

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