血管内皮生长因子、碱性成纤维细胞生长因子和内皮抑素在肝硬化中的表达及意义

doi:10.3969/j.issn.1004-583X.2018.05.016

临床荟萃

血管内皮生长因子、碱性成纤维细胞生长因子和内皮抑素在肝硬化中的表达及意义

河北医科大学第二医院 a.肝胆外科；b.肿瘤外科，河北石家庄 050000

出版日期:2018-05-05 发布日期:2018-05-29
通讯作者: 通信作者：闫长青，Email：docyan001@sian.com
基金资助:
河北省自然科学基金资助项目（H2015206510）

Experssion and significance of VEGF， bFGF， endostatin in hepatic sclerosis

a.Department of Hepatobilary Surgery；b. Department of Surgical Oncology，
the Second Hospital of Hebei Medical University， Shijiazhuang 050000， China

Online:2018-05-05 Published:2018-05-29
Contact: Corresponding author: Yan Changqing，Email：docyan001@sian.com

摘要/Abstract

摘要： 目的探究血管内皮生长因子（vascular endothelial growth factor， VEGF）、碱性成纤维细胞生长因子（basic fibroblast growth factor， bFGF）和内皮抑素（endostatin）在肝硬化中的表达情况。方法收集从2015年1月至2016年6月河北医科大学第二医院肝胆外科收治的肝硬化患者22例，记为实验组；另选同期10例正常肝脏组织作为正常组。分别用HE和Masson三色染色观察两组标本；用免疫组织化学和酶联免疫吸附方法(ELISA)检测两组中VEGF，bFGF，Endostatin蛋白表达。结果 ①HE染色和Masson三色染色结果显示：与正常组相比，实验组镜下肝脏组织中纤维条索明显增多，有明显的假小叶生成，其间广泛分布炎性细胞，汇管区结构明显消失，肝细胞有不同程度地坏死，纤维增生也更加明显；②免疫组织化学：正常组VEGF，bFGF和Endostatin的蛋白相对灰度值分别为0.06±0.03，0.08±0.05和0.07±0.02；实验组VEGF，bFGF和Endostatin的相对灰度值分别为0.16±0.02，0.14±0.03和0.14±0.01；与正常组相比，实验组VEGF，bFGF和Endostatin表达明显上调，且差异均有统计学意义（P<0.05）。③ ELISA试验：正常组VEGF，bFGF和Endostatin的吸光度（OD值）分别为283.05±14.32，30.03±2.11和4 350.63±636.66；实验组VEGF，bFGF和Endostatin的OD值分别为539.63±160.28，63.99±33.28和8 194.75±1 319.28；与正常组相比，实验组VEGF，bFGF和Endostatin表达均明显上调，且差异均有统计学意义（P<0.05）。结论肝硬化患者VEGF，bFGF和Endostatin蛋白表达均明显上调，这些蛋白的表达与肝硬化发生发展密切相关。

关键词: 肝硬化, 血管内皮生长因子, 成纤维细胞生长因子, 内皮抑素

Abstract: Objective To explore the expressions of vascular endothelial growth factor (VEGF)， basic fibroblast growth factor (bFGF) and endostatin in hepatic sclerosis. Methods The data of 22 patients with hepatic sclerosis who admitted to our hospital from January 2015 to June 2016 were analyzed and named as experience group;, while 10 cases of normal liver tissues were selected as the normal group. HE and Masson trichrome staining were used to observe the two groups of specimens. The expression of VEGF， bFGF and Endostatin in the experimental group and the normal group were detected by immunohistochemistry and ELISA. Results ① HE staining and Masson staining showed that the experimental group, compared with normal group，witnesses the significant increase of the fiber bands， the formation of false lobule，the expansion of a large number of inflammatory cells and the disappearence of the structure of the portal area in the liver tissue. ② As to the immunohistochemistry, the relative gray level values of VEGF， bFGF and endostatin in normal group were 0.06±0.03，0.08±0.05 and 0.07±0.02 respectively;while the relative gray level values of VEGF， bFGF and endostatin in experimental group were 0.16±0.02， 0.14±0.03 and 0.14±0.01 respectively. Compared with the normal group， the expression of VEGF， bFGF and endostatin were significantly upregulated in the experimental group (P<0.05). ③ In ELISA experiment, the absorbance (OD) of VEGF, bFGF and endostatin in normal group were 283.05±14.32，30.03±2.11 and 4 350.63±636.66 respectively; The absorbance (OD) of VEGF， bFGF and endostatin in experimental group were 539.63±160.28， 63.99±33.28 and 8 194.75±1 319.28, respectively. Compared with the normal group, the expression of VEGF， bFGF and endostatin were significantly upregulated in the experimental group. Conclusion The expression of VEGF, bFGF and endostatin protein are upregulated in patients with hepatic sclerosis. The expression of these proteins is closely related to the occurrence and development of the disease.

Key words: liver , cirrhosis；vascular , endothelial , growth , factor；fibroblast , growth , factor；endostatins

段佳悦a，史清峰a，王明光a，戚诚b，闫长青a. 血管内皮生长因子、碱性成纤维细胞生长因子和内皮抑素在肝硬化中的表达及意义[J]. 临床荟萃, doi: 10.3969/j.issn.1004-583X.2018.05.016.

Duan Jiayuea， Shi Qingfenga， Wang Mingguanga， Qi Chengb， Yan Changqinga. Experssion and significance of VEGF， bFGF， endostatin in hepatic sclerosis[J]. Clinical Focus, doi: 10.3969/j.issn.1004-583X.2018.05.016.

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链接本文: https://huicui.hebmu.edu.cn/CN/10.3969/j.issn.1004-583X.2018.05.016

https://huicui.hebmu.edu.cn/CN/Y2018/V33/I5/427

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血管内皮生长因子、碱性成纤维细胞生长因子和内皮抑素在肝硬化中的表达及意义

Experssion and significance of VEGF， bFGF， endostatin in hepatic sclerosis

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